Bertin Health & Life Sciences

WHITE PAPER

Best practices for cryogrinding
with the Precellys Evolution

 

Cryogrinding, also known as freeze powdering, cryogenic grinding, freezer milling, and cryomilling, is the act of turning a deep-frozen sample into a powder in a temperature-controlled environment. Cryogenic grinding is one of the most effective methods to extract DNA, RNA, proteins, and metabolites from a biological sample. An inadequate mechanical sample disruption protocol can lead to nucleic acid shearing or protein degradation. Proteins and RNA are thermosensitive molecules that can be degraded by the heat generated during mechanical homogenization. 

In this white paper, we show how cryogrinding can be performed using the Precellys Evolution in combination with the Cryolys Evolution cooling module and the Precellys stainless steel metal tubes. We offer examples of protocols to efficiently pulverize deep-frozen animal and vegetable samples and maximize the recovery of nucleic acids, proteins, and metabolites.


 

All you need to know about:
• Freeze powdering using Cryolys® Evolution
• Precellys Evolution: Whole mouse brain Cryogrinding



 

You may also be interested in

[White Paper] Cryogrinding - Precellys

WHITE PAPER
Best practices in mouse tissue RNA Extraction with Precellys Homogenizers

 

WHITE PAPER
Best practices for DNA sample preparation with Precellys Homogenizers

 

WHITE PAPER
Best practices in sample preparation for reliable sequencing results with Precellys Homogenizers

Bertin Health & Life Sciences

Your expert in life sciences application

   Follow us on Linkedin!Follow us on Youtube!