DNA extraction is a method to purify DNA by using physical and/or chemical methods from a sample, separating DNA from cell membranes, proteins, and other cellular components. A large number of techniques have been developed since the first DNA extraction was carried out by Friedrich Miescher in 1869. Nonetheless, there are five basic steps of DNA extraction that are consistent across all the possible DNA purification chemistries: 1) cellular lyses, 2) separation of the soluble DNA from cell debris, 3) binding the DNA of interest to a purification matrix, 4) washing proteins and other contaminants away from the matrix and 5) elution of the DNA
With today’s requirements for DNA analyses by multiplex, real time PCR and NGS, the importance of high-quality, purified DNA cannot be underestimated. Various factors, such as tissue type and DNA integrity, must be considered for choosing the appropriate DNA extraction method. Precellys homogenizers play a key role at the beginning of all the workflows requiring a biological sample homogenization for molecule extraction as a first step.
In this White Paper we have listed some tips and tricks as well as some case studies of how to use your Precellys homogenizer to optimize the separation of the soluble DNA from your samples.