[Application note]

Use of the Precellys® Evolution homogenizer for unbiased DNA extraction from the ZymoBIOMICS™ Microbial Community Standard

Microbiome profiling via Next Generation Sequencing (NGS) techniques is rapidly changing the landscape of microbiology. However, to ensure accurate microbiome measurements, it is essential to ensure the microbiome workflow is unbiased and robust. The ZymoBIOMICS™ Microbial Community is a mock microbial community standard comprised of ten well-characterized organisms (i.e., 5 gram-positive, 3 gram-negative, and 2 yeast) that are mixed at defined proportions. The standard contains microbes of different cell wall recalcitrance and of different cell size making it ideal for evaluating cell lysis efficiency. In this study, the efficiency of microbial lysis with the ZymoBIOMICS™ Microbial Community Standard was assessed using the Precellys® Evolution tissue homogenizer combined with Cryolys® Evolution cooling unit (Bertin Instruments).