The biggest challenge when working with fibrous, elastic, and tough samples is efficient sample processing. The presence of elastic tissue, calcium phosphate and other minerals can develop into a solid and tough matrix which can be difficult to homogenize.
Moreover, heat generation inside the lysing kit tubes during the process results in degradation and denaturation of proteins and RNA. Heat generation is even more severe when working with harder samples that require higher energy for efficient homogenization, resulting in a more challenging process.
In this white paper, discover a selection of examples and protocols of the use of the Precellys Evolution homogenizer together with the Cryolys Evolution cooling unit for the homogenization of hard samples within minutes and without degradation.