[Application note]

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the application note on 20-Hydroxyecdysone assessment in decapoda crustacean (arthropods)

Endocrine disruptors (EDCs) are present in the environment, coming from several origins (industrial products, pesticides or even hormones). When accumulated in organisms, EDCs can lead to endocrine system dysfunctions and consequently to population decline, which could in long term impact the ecosystem's functioning. Although in vertebrates EDC biomarkers are well established (e.g. vitellogenin). Few are available in invertebrates, such as arthropods (e.g. strings or insects). This work intended to study the impact of the insecticide chlordecone (CLD), widely used in French West Indies until 1993, on the concentration of the 20-hydroxyecdysone (20-HE, key hormone of the arthropod endocrine system), in the decapod Macrobrachium rosenbergii (in vivo).

This study is the first to highlight the impact of a CLD exposure on the hormonal system of the commercial prawn, Macrobrachium rosenbergii, by measuring the 20-HE concentration. The results allowed to conclude that CLD could have an anti-ecdysteroidal activity, as suggested for other estrogeno-mimetic EDCs, and it could have a long term impact on the physiology of decapods by disrupting their molt cycle, and consequently their growth. The use of the Precellys homogenizer ensures a high extraction yield and a protocol reproductibility. Combined with Bertin Bioreagent's ELISA Kit, it allows to accurately measure the impact of CLD.